Abstract
Methanosarcina barkeri was grown on trimethylamine, methylamine, or methanol containing completely deuterated methyl groups. Methane was collected and analyzed in a mass spectrometer. It contained 79 to 83% CD3H and 14 to 18% CD2H2. This demonstrated that the methyl groups of the above compounds served primarily as direct precursors of methane.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hermans J. M., Hutten T. J., van der Drift C., Vogels G. D. Analysis of coenzyme m (2-mercaptoethanesulfonic acid) derivatives by isotachophoresis. Anal Biochem. 1980 Aug;106(2):363–366. doi: 10.1016/0003-2697(80)90533-3. [DOI] [PubMed] [Google Scholar]
- Hippe H., Caspari D., Fiebig K., Gottschalk G. Utilization of trimethylamine and other N-methyl compounds for growth and methane formation by Methanosarcina barkeri. Proc Natl Acad Sci U S A. 1979 Jan;76(1):494–498. doi: 10.1073/pnas.76.1.494. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PINE M. J., BARKER H. A. Studies on the methane fermentation. XII. The pathway of hydrogen in the acetate fermentation. J Bacteriol. 1956 Jun;71(6):644–648. doi: 10.1128/jb.71.6.644-648.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PINE M. J., VISHNIAC W. The methane fermentations of acetate and methanol. J Bacteriol. 1957 Jun;73(6):736–742. doi: 10.1128/jb.73.6.736-742.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shapiro S., Wolfe R. S. Methyl-coenzyme M, an intermediate in methanogenic dissimilation of C1 compounds by Methanosarcina barkeri. J Bacteriol. 1980 Feb;141(2):728–734. doi: 10.1128/jb.141.2.728-734.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weimer P. J., Zeikus J. G. One carbon metabolism in methanogenic bacteria. Cellular characterization and growth of Methanosarcina barkeri. Arch Microbiol. 1978 Oct 4;119(1):49–57. doi: 10.1007/BF00407927. [DOI] [PubMed] [Google Scholar]