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. 1998 Sep 15;95(19):11241–11245. doi: 10.1073/pnas.95.19.11241

Figure 4.

Figure 4

Overexpression of Rss1/Gle1 partially rescues the ts phenotype seen in NUP82-Δ108U yeast. The (+) rows contain NUP82-Δ108U yeast harboring pVDP7 (which overexpresses Rss1/Gle1). The (−) rows contain NUP82-Δ108U yeast harboring pRS315 (empty plasmid). Cells were grown in selective medium (-LEU) at 30°C overnight. Equivalent numbers of cells and serial 10-fold dilutions (based on OD600) were plated on either complete medium (YPD) or selective medium (-LEU) and incubated at 30°C or 37°C for 4 days.