Skip to main content
. 1998 Sep 15;95(19):11251–11256. doi: 10.1073/pnas.95.19.11251

Figure 6.

Figure 6

Expression of Mix family proteins and markers of endoderm differentiation in animal pole explants and whole embryos. (A) Expression of Mix family members leads to the induction of endoderm. Embryos were injected at the one-cell stage with 1 ng of each RNA. Smad1 and M11 are included as negative controls. Animal pole explants were dissected at stage 8 and cultured to sibling stage 36. RT-PCR analysis demonstrated that Mix.3 and Mix.4 induce endodermal markers edd, IFABP, GATA-5, XSox17α, and XlHbox8. Overexpression of Mix family members, without added growth factors, failed to induce mesoderm markers such as globin. (B) Whole embryo in situ hybridization for edd. Embryos were injected with Mix RNA (500 pg/blastomere) at the two-cell stage, cultured to sibling control stage 38, and then fixed and stained for edd expression by in situ hybridization. Mix.3 led to ectopic edd expression (54/55 injected embryos) whereas Mix.1 did not (0/37 injected embryos).