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Purification of the pheromone by normal-phase HPLC. A cyclopentane solution of pheromone (550 μl; derived from ca. 4 × 1013 cells and prepurified by reversed-phase HPLC) was loaded onto a Nucleosil NH2 column using a 1-ml sample loop and eluted at 1 ml/min with a gradient (dashed line) of methyl acetate in cyclopentane. Fractions with pheromone activity are indicated by a bar.
