Figure 4.

Unrooted maximum parsimony tree of several archaeal and bacterial tRNA^Lys sequences, in the context of sequences from all 20 tRNA types from E. coli, aligned without their anticodon triplets (tRNAs other than tRNA^Lys are indicated by the three-letter code of their corresponding amino acid). Numbers at nodes of the tRNA^Lys branches correspond to bootstrapping frequencies for the branch closest to the number (calculated from 100 different trees). The tRNA^Lys sequences, charged by class I or II LysRS, are boxed, and those known to be charged by a class I LysRS are marked (I). S. aureus, Staphylococcus aureus; B. subtilis, Bacillus subtilis; H. influenzae, Haemophilus influenzae; H. volcanii, Halobacterium volcanii; M. kandleri, Methanopyrus kandleri; M. thermo., Methanobacterium thermoautotrophicum; M. fervidus, Methanothermus fervidus; M. voltae, Methanococcus voltae; M. vannielii, Methanococcus vannielii.