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Regulation of cyclin D, p21, and p27 by the ubiquitin-dependent proteolysis. (A) Log-phase RKO or HaCat cells were treated with either dimethyl sulfoxide (DMSO) (0.05%), 10 μg/ml of LLNL, or N-acetyl-l-leucinyl-l-leucinyl-methional (LLM) for 6 hr. The cyclin D, p21, and p27 protein were isolated and detected by their respective antibodies by using immunoprecipitation and Western blot analyses.
