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Expression of recombinant attractin and immunoprecipitation by antibody against natural attractin. (A) In vitro transcription/translation of attractin in absence or presence of glycosyl transferases. (B) Western blotting with anti-myc of lysates of 293T cells transiently transfected with pSecTag2B-attractin or pSecTag2B vector control. (C) Attractin was precipitated with preimmune antibody or antiattractin antibody from lysates of CHO cells stably transfected with pSecTag2B-attractin.
