Expression of IL-2Rα, IL-2Rβ, and IL-2Rγ by CD4 T lymphocytes of HIV-infected patients. PBMC were treated by mAbs 33B3, CF1, 3B5, or IOT28 (anti-CD28). Characterization of the population was achieved by treatment with anti-CD3 mAb (data not shown). FITC-labeled Fab fragment anti-IgG was used, followed by PE-conjugated anti-CD4 + TC-conjugated anti-CD14 mAbs. (A) IL-2R expression of a representative group 1 patient. The percentage of CD4 T lymphocytes positive for the different IL-2R chains is indicated. (B) Expression of IL-2Rα, IL-2Rβ, and IL-2Rγ by CD4 T lymphocytes of healthy donors (HIV−, n = 11), group 1 patients (G1, n = 9), and group 2 (TCT) patients (G2, n = 10). Mean numbers of positive cells (±SD) are shown. P values are indicated as in Fig. 1.