Figure 1.
Determination of IL-3 transgene integration and expression in F1 intermating mice. (A) mRNA from various tissues of IL3-Tg (TG) and controls (CT) were analyzed by RT-PCR for IL-3 expression by using specific primers. The RT-PCR product was loaded onto an agarose gel, transferred onto nylon membrane and probed with a radiolabeled-specific IL-3 probe. (B) The level of IL-3 protein expression in various organs of IL3-Tg (TG) or controls (CT) was determined by ELISA. Data from four mice per group are expressed as mean ± SD.