Table 1.

Loss of MN in the spinal cord of IL3-Tg mice

Mice	Number of sections	Staining	MN count (mean ± SD)
CT	50	Nissl	47.9  ±  8
IL3-Tg	27	Nissl	31.3  ±  6.1
CT	28	ChAT	36.2  ±  4.5
IL3-Tg	19	ChAT	19.5  ±  6.2

Coronal sections from the lumbar spinal cord (L4–L5) of four 10-month-old transgenic (IL3-Tg) and four age-matched controls (CT) were either immunolabeled with ChAT antibodies or stained with the Nissl dye. The total number of sections used for the determination of MN number ± SD is indicated. Both Nissl and ChAT staining showed significant differences in MN counts between IL3-Tg and controls, using the Student’s t test (P < 0.05 for Nissl values; P < 0.001 for ChAT values).