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. 2007 Oct 26;4:112. doi: 10.1186/1743-422X-4-112

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Copyright © 2007 Geiss et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Packaging and infection of pBG78 replicons in BHK and C6/36 cells. pBG78 PIPS were generated by co-transfecting pBG78 and pBG44 plasmid DNA into BHK cells. Media was collected 24 hrs post transfection and 100 ul of the clarified media was added to new BHK or C6/36 cells. 24 hrs after infection the media was replaced with fresh media containing10 ug/ml blasticidin and the cultures were maintained for an additional three weeks. Media was replaced every 3–4 days. GFP fluorescence was detected at 3 days post infection (panels B and E) and 3 weeks post infection (panels D and H).