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. 2007 Oct 26;4:112. doi: 10.1186/1743-422X-4-112

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Copyright © 2007 Geiss et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SINV replicons can express epitope tagged mosquito genes for several days. A. C6/36, S2, or BHK cells were infected with PIPs expressing a V5 epitope-tagged Ae. aegypti R2D2 cDNA either in a sense (pBG156) or antisense (pBG155) orientation. Cells were treated with blasticidin for four days and V5-R2D2 detected by western blot analysis. B. C6/36 cells were co-cultured with pBG156/pBG44 co-transfected BHK cells, transferred to new plates, and samples of cells collected at the indicated times for western blot analysis.