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. 2007 Oct 30;4:116. doi: 10.1186/1743-422X-4-116

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Copyright © 2007 Gruener et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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HPV16 E5 forms a ternary complex with calnexin and the HLA-I heavy chain. HeLa cells were transiently transfected with AU1-tagged codon-optimised HPV16 E5, M1, or empty vector. 24 h later CHAPS lysates were immunoprecipitated with antibodies against the E5-tag (anti-AU1). Precipitated immune complexes were separated by SDS-PAGE and Western blotted using anti-calnexin and anti-HLA-B, -C mAb (HC10), respectively (band marked with *). Molecular-mass markers in kDa are indicated at the left of the blots.