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. 1999 Oct 4;147(1):33–44. doi: 10.1083/jcb.147.1.33

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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SPP has a different affinity for the products generated during processing. Aliquots of processing reactions with immobilized SPP using preFD and preHSP21 as substrates were collected at intervals over a 60-min period and immediately separated into the supernatant and the immobilized SPP fraction before analysis by standard SDS-PAGE (a) or tricine SDS-PAGE (b–e). [³⁵S]cysteine-labeled preFD, used to monitor generation of mature FD, supernatant (a). [³⁵S]methionine-labeled preFD, used to monitor generation of FD transit peptide and its subfragment, supernatant (b), and immobilized SPP fraction (c). [³⁵S]methionine-labeled preHSP21 supernatant (d), and immobilized SPP fraction (e). Substrate control (C), lane 1.