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. 1999 Oct 4;147(1):59–70. doi: 10.1083/jcb.147.1.59

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Quantitative analysis of neurotrophin stimulated β-actin mRNA localization. Neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), or NGF was added to the MEM (25 ng/ml) for the indicated time. NT-3 and BDNF, but not NGF, were observed to rapidly stimulate the localization of β-actin mRNA within growth cones. The axon-like neurite and growth cone from each cell was visualized for the presence of β-actin mRNA granules (see Materials and Methods). 100 cells were scored per coverslip. Histogram shows mean percentage and standard deviation between independent samples (n = 4). Examples of localized and nonlocalized cells are shown in Fig. 1. N2, normal control. MEM, starvation in minimum essential medium. *, P < 0.01 when MEM compared with N2. #, P < 0.01 when NT-3 or BDNF was compared with MEM.