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. 1999 Oct 4;147(1):59–70. doi: 10.1083/jcb.147.1.59

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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NT-3 stimulated localization of β-actin mRNA and protein analyzed using quantitative digital imaging microscopy. Neurons were fixed for in situ hybridization to β-actin mRNA (A) and immunofluorescence detection of β-actin protein (B). DIC and fluorescence images were captured using a cooled CCD camera. 20 growth cones were imaged for each condition with identical exposure times. Data expressed as fluorescence density (total intensity/growth cone area). NT-3 was observed to increase the density of fluorescence signal for both β-actin mRNA and protein within growth cones. #, P < 0.01 when MEM was compared with N2, or MEM was compared with NT-3, 10 min or NT-3, 2 h. *, P < 0.05 when MEM was compared to NT-3 at 10 min. N2, normal culture medium. MEM, starvation in minimum essential medium.

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