Figure 5.
Differential effects of cytochalasin-D and colchicine on NT-3–stimulated β-actin mRNA localization. Cytochalasin-D (5 μg/ml) or colchicine (10 μg/ml) was added to the MEM for 30 min before NT-3 stimulation. Fluorescence intensity was measured using digital imaging microscopy and divided by growth cone area. Colchicine treatment, but not cytochalasin-D, inhibited the localization of β-actin mRNA by NT-3. *, P < 0.05 when NT-3 at 10 min was compared with MEM. #, P < 0.01 when cytochalasin-D/NT-3 at 10 min was compared with MEM.