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. 1999 Oct 4;147(1):59–70. doi: 10.1083/jcb.147.1.59

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Time course of β-actin mRNA and protein localization to growth cones after NT-3 treatment. Neurons were cultured for 4 d and starved in MEM for 6 h. NT-3 (25 ng/ml) was added to the medium for indicated time, and cells were fixed for in situ hybridization and immunofluorescence analysis. A rapid increase in β-actin protein localization (2 min) was observed before maximal localization of β-actin mRNA within growth cones (10 min).