Figure 1.
TSST-1-induced MIF production by mouse macrophages. RAW 264.7 macrophages (3 × 106 cells) were stimulated for 12 hr with TSST-1 at the indicated concentrations. The content of MIF secreted into the medium was analyzed by Western blotting as described in Materials and Methods. Mouse recombinant MIF (10 ng) was electrophoresed and transferred as a standard. MIF concentrations in cell-conditioned media also were quantified by ELISA. Samples were analyzed in triplicate and results expressed as mean ± SEM of three separate experiments. MIF concentrations were 204 ± 149 pg/ml (control cells), and 396 ± 307 pg/ml, 1,694 ± 467 pg/ml, 1,156 ± 380 pg/ml, 1,009 ± 61 pg/ml, 410 ± 282 pg/ml and 321 ± 297 pg/ml in supernatants of cells induced with 10−3, 10−2, 10−1, 1, 10, and 102 ng/ml of TSST-1, respectively.