Table 1.
Concentration of TSST-1, ng/ml | Macrophage MIF production, pg/ml
|
|
---|---|---|
Mean | SEM | |
0 | 130 | 130 |
0.001 | 135 | 135 |
0.01 | 626 | 360 |
0.1 | 259 | 7 |
1 | 340 | 74 |
10 | 215 | 215 |
100 | 146 | 146 |
Thioglycollate-elicited peritoneal macrophages (2 × 106 cells) were incubated for 12–14 hr with TSST-1 at the indicated concentrations and the MIF content of the cell-conditioned media was analyzed by ELISA. Samples were analyzed in triplicate and results expressed as mean ± SEM of two separate experiments.