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. 1998 Sep 15;95(19):11394–11399. doi: 10.1073/pnas.95.19.11394

Table 1.

Choline growth phenotype and enzyme activities in bet mutants

Strain (genotype) Growth* Enzyme activity
CDH BADH MDH
102F34R (wild type) 100 113 ± 22 84 ± 17 214 ± 8
UNA130 (betA2∷Ω) NG ND 61 ± 12 214 ± 3
UNA156 (betB2∷Ω) NG ND ND 213 ± 2
UNA208 (betC4∷Tn5) NG ND ND 185 ± 31
UNA210 (betI5∷Tn5) 20 18 ± 2 3 ± 1 186 ± 20
UNA137 (betCBAΔΩ) NG ND ND 190 ± 7

NG, no growth improvement compared with LAS + 0.5 M NaCl (<10% wild-type growth). ND, not detected (CDH, <10 nmol/min per mg; BADH, <1 nmol/min per mg). 

*

The ability of the strains to use choline as osmoprotectant was determined in LAS + 0.5 M NaCl + 1 mM choline. Values represent the percentage of wild-type growth. 

Cells were grown in MCAA + choline. Enzyme activity was determined from triplicate assays and given in nmol/min per mg of protein ± SEM.