Table 1.

Choline growth phenotype and enzyme activities in bet mutants

Strain (genotype)	Growth*	Enzyme activity†
		CDH	BADH	MDH
102F34R (wild type)	100	113 ± 22	84 ± 17	214 ± 8
UNA130 (betA2∷Ω)	NG	ND	61 ± 12	214 ± 3
UNA156 (betB2∷Ω)	NG	ND	ND	213 ± 2
UNA208 (betC4∷Tn5)	NG	ND	ND	185 ± 31
UNA210 (betI5∷Tn5)	20	18 ± 2	3 ± 1	186 ± 20
UNA137 (betCBAΔΩ)	NG	ND	ND	190 ± 7

NG, no growth improvement compared with LAS + 0.5 M NaCl (<10% wild-type growth). ND, not detected (CDH, <10 nmol/min per mg; BADH, <1 nmol/min per mg). 

^*The ability of the strains to use choline as osmoprotectant was determined in LAS + 0.5 M NaCl + 1 mM choline. Values represent the percentage of wild-type growth. 

^†Cells were grown in MCAA + choline. Enzyme activity was determined from triplicate assays and given in nmol/min per mg of protein ± SEM.