Abstract
A new relaxed mutant of Bacillus subtilis was isolated by screening Rifr clones for alterations in stringent control. The Rifr relaxed mutant which is described was found to contain a second-site mutation conferring a relaxed response to an energy source downshift and was partially relaxed after amino acid starvation. The new rel locus, called relG, was distinct from the two other known rel loci in B. subtilis, relA, and relC.
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- Atherly A. G. Escherichia coli mutant containing a large deletion from relA to argA. J Bacteriol. 1979 May;138(2):530–534. doi: 10.1128/jb.138.2.530-534.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Friesen J. D., An G., Fiil N. P. Nonsense and insertion mutants in the relA gene of E. coli: cloning relA. Cell. 1978 Dec;15(4):1187–1197. doi: 10.1016/0092-8674(78)90045-4. [DOI] [PubMed] [Google Scholar]
- Gallant J. A. Stringent control in E. coli. Annu Rev Genet. 1979;13:393–415. doi: 10.1146/annurev.ge.13.120179.002141. [DOI] [PubMed] [Google Scholar]
- Gallant J., Palmer L., Pao C. C. Anomalous synthesis of ppGpp in growing cells. Cell. 1977 May;11(1):181–185. doi: 10.1016/0092-8674(77)90329-4. [DOI] [PubMed] [Google Scholar]
- Halling S. M., Burtis K. C., Doi R. H. Reconstitution studies show that rifampicin resistance is determined by the largest polypeptide of Bacillus subtilis RNA polymerase. J Biol Chem. 1977 Dec 25;252(24):9024–9031. [PubMed] [Google Scholar]
- Haworth S. R., Brown L. R. Genetic analysis of ribonucleic acid polymerase mutants of Bacillus subtilis. J Bacteriol. 1973 Apr;114(1):103–113. doi: 10.1128/jb.114.1.103-113.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nierlich D. P. Regulation of bacterial growth, RNA, and protein synthesis. Annu Rev Microbiol. 1978;32:393–432. doi: 10.1146/annurev.mi.32.100178.002141. [DOI] [PubMed] [Google Scholar]
- Nishino T., Gallant J., Shalit P., Palmer L., Wehr T. Regulatory nucleotides involved in the Rel function of Bacillus subtilis. J Bacteriol. 1979 Nov;140(2):671–679. doi: 10.1128/jb.140.2.671-679.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pao C. C., Gallant J. A gene involved in the metabolic control of ppGpp synthesis. Mol Gen Genet. 1978 Jan 17;158(3):271–277. doi: 10.1007/BF00267198. [DOI] [PubMed] [Google Scholar]
- Price V. L., Brown L. R. Transcriptional inhibition and production of guanosine polyphosphates in Bacillus subtilis. J Bacteriol. 1981 Sep;147(3):752–756. doi: 10.1128/jb.147.3.752-756.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith I., Paress P., Cabane K., Dubnau E. Genetics and physiology of the rel system of Bacillus subtilis. Mol Gen Genet. 1980;178(2):271–279. doi: 10.1007/BF00270472. [DOI] [PubMed] [Google Scholar]
- Smith I., Paress P., Pestka S. Thiostrepton-resistant mutants exhibit relaxed synthesis of RNA. Proc Natl Acad Sci U S A. 1978 Dec;75(12):5993–5997. doi: 10.1073/pnas.75.12.5993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stephens J. C., Artz S. W., Ames B. N. Guanosine 5'-diphosphate 3'-diphosphate (ppGpp): positive effector for histidine operon transcription and general signal for amino-acid deficiency. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4389–4393. doi: 10.1073/pnas.72.11.4389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swanton M., Edlin G. Isolation and characterization of an RNA relaxed mutant of B. subtilis. Biochem Biophys Res Commun. 1972 Jan 31;46(2):583–588. doi: 10.1016/s0006-291x(72)80179-7. [DOI] [PubMed] [Google Scholar]
- Uzan M., Danchin A. A rapid test for the rel A mutation in E. coli. Biochem Biophys Res Commun. 1976 Apr 5;69(3):751–758. doi: 10.1016/0006-291x(76)90939-6. [DOI] [PubMed] [Google Scholar]
- Weinstein B. I., Bhardwaj N., Li H. C. A rapid and accurate procedure for assaying DNA polymerase, RNA polymerase, or ribosome dependent protein synthesis. Anal Biochem. 1975 Sep;68(1):62–69. doi: 10.1016/0003-2697(75)90679-x. [DOI] [PubMed] [Google Scholar]