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. 1998 Sep 15;95(19):11412–11417. doi: 10.1073/pnas.95.19.11412

Figure 2.

Figure 2

BDNF and estradiol have opposing effects on GAD immunoreactivity. (a and b) The number of GAD-positive fluorescent particles decreased significantly after 24 hr of estradiol treatment [control, 55.9 ± 15.5 units (a); estradiol, 5.6 ± 1.5 units (b)]. (c) GAD immunoreactive levels increased significantly with BDNF treatment (227.3 ± 17.6). (d) Cultures exposed to BDNF and estradiol simultaneously did not exhibit decreased GAD staining seen with estradiol alone (83.6 ± 19). (Bar = 10 μm.) Similar results were obtained with GABA staining (images not shown).