BDNF-AS mimics the estradiol response. Cultures were dosed in serum free media for 2 days. (a) Control. (b) Estradiol. (c) RAS. (d) BDNF-AS. Images are reconstructions of z-series from single 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine-labeled neurons, taken on the confocal laser-scanning microscope. (Bar = 10 μm.)