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Quantification of the SMOD of BDNF in various song system nuclei as a function of age. In HVc, the moderate SMOD of a 20-day-old male is attributable to the many stained cells within the nucleus (Upper Left). The SMOD peaks in HVc at around 45 days of age. The other song system areas of RA, LMAN, and area X show peak levels of BDNF SMOD at a much later time of ≈65 days of age. The SMOD values for all of these song system areas drops sharply in the adult male (Lower Right).
