Figure 6.

Immunoblot to determine level of lycopene β-cyclase. An increase is observed by 24 h in Car-3 mutant cells (B), which also have a much greater carotenoid accumulation than in wild-type cells (A). Each lane of the SDS/polyacrylamide gel was loaded with 1.2 μg chlorophyll. Membranes were probed with an antibody prepared to a recombinant protein of a gene encoding lycopene β-cyclase (LYCB) of Arabidopsis (as in methods of ref. 17). Protein level of rubisco was used as a control, and bound antibody was visualized by enhanced chemiluminescence.