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MB structure. MBs were synthesized with a 24-nt “loop” sequence flanked on the 5′ and 3′ ends with complementary sequence 5 nt long. Internal hybridization of the complementary ends creates the stem–loop structure. The fluorescent donor (EDANS) and acceptor (DABCYL) molecules are joined to the 5′ terminal phosphate and the 3′ terminal hydroxyl group, respectively, through (CH2)6-S-CH2-CO and (CH2)7-NH linker arms.
