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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Mol Reprod Dev. 2008 Feb;75(2):345–354. doi: 10.1002/mrd.20769

Fig. 2.

Fig. 2

Structural integrity of grade I immature oocytes (bright field microscopy; A,C,E,G,I) and cumulus–oocyte communications (micro-injection and diffusion of fluorescent Luciferin Yellow; B,D,F,H,J) after exposure to anisosmotic and isosmotic solutions. A,B: Cumulus–oocyte complex (COC) with open communications after exposure to hypo- or isosmotic condition. C,D: COC with partially open communications after exposure to 1,000 mOsm. E,F: COC with closed communications after exposure to 2,000 mOsm. G,H: COC with open communications after treatment with cytochalasin B and exposure to 1,000 mOsm. I,J: COC with partially open communications after treatment with cytochalasin B and exposure to 2,000 mOsm. Bar = 100 μm. [See color version online at www.interscience.wiley.com.]