TABLE 4.
Incidences of Nuclear Maturation, Success of Fertilization, and Embryo Cleavage After Exposure of Immature Oocytes to Anisosmotic versus Isosmotic (290 mOsm) Solutions in the Presence or Absence of Cytochalasin B (CB)
| Exposure (mOsm) | Metaphase I (%)a | Metaphase II (%)a | Fertilized (%)b | Cleaved (%)b |
|---|---|---|---|---|
| 100 | 25.0 b | 62.5 b | 56.7 b | 51.7 b |
| 100 + CB | 25.0 b | 63.3 b | 54.2 b | 50.8 b |
| 290 | 8.3 a | 85.8 a | 75.0 a | 72.5 a |
| 290 + CB | 7.5 a | 84.2 a | 73.3 a | 73.3 a |
| 1,000 | 10.0 a | 80.8 a | 72.5 a | 70.8 a |
| 1,000 + CB | 9.2 a | 80.0 a | 71.7 a | 70.0 a |
| 1,500 | 29.2 b | 57.5 b | 53.3 b | 50.0 b |
| 1,500 + CB | 11.7 a | 79.2 a | 74.2 a | 70.0 a |
| 2,000 | 30.8 b | 55.8 b | 50.8 b | 44.2 b |
| 2,000 + CB | 12.5 a | 78.3 a | 72.5 a | 69.2 a |
Within each column values with different letters differ (P < 0.05).
a
Percentage relative to 120 total oocytes/exposure condition fixed after IVM.
b
Percentage relative to 120 total oocytes/exposure condition observed at 48 hpi.