Table 1.

Inhibition of telomerase by 2′-O-methyl RNA, PNA, and DNA oligomers and melting temperatures to complementary RNA

Sequence	IC50, μM		Tm, °C
	23°C	37°C
3′-GAGUCAAUCCCAAUCUG-5′ (RNA template)
1   CAGUUAGGGUUAG	0.002	0.008	58
1d  CAGTTAGGGTTAG (DNA)	12	ND	46
1p  CAGTTAGGGTTAG (PNA)	0.01	0.02	76
2   GACUUAGAAUUAG (mismatch)	>33	ND	38
3   GAGUUAGGG	0.02	0.2	53
4    AGUUAGGG	0.03	1.0	47
5     GUUAGGG	0.3	0.7	35
8      UUAGGG	0.3	11	28
7   CAGUUAGGGUUAG	0.002	0.003	59
8   CAGUUAGAAUUAG (mismatch)	0.6	3.0	34
9   CAGUUAGGGUUAG	0.007	0.03	56
10  CAGUUAGAAUUAG(mismatch)	0.07	0.2	29
11  UGACUGUGAUGGA(mixed)	0.1	0.7	ND

All oligomers are 2′-O-meRNA unless otherwise noted. The PNA is listed N to C termini. ND, not detectable. Underlined nucleotides have phosphorothioate linkages. Reported melting temperatures are ±1°C or less.