. 2007 Oct 15;51(12):4486–4488. doi: 10.1128/AAC.01462-06

TABLE 1.

Primers used for PCRs

Amplified DNA	Primer	Oligonucleotide sequence (5′ to 3′)	Accession no.
Variable region of class 1 integrons	L1	`GGCATCCAAGCAGCAAGC`	U49101
	R1	`AAGCAGACTTGACCTGAT`
intI1	Int-IN	`TGTCGTTTTCAGAAGACGG`	U49101
	IntA-R	`ATCATCGTCGTAGAGACG`
	IntB-F	`GTCAAGGTTCTGGACCAG`
	Int-out-F	`GTAGAACAAGCAGGCATC`
	Int-out-R	`GAAACGGATGAAGGCACG`
aac(6′)-Ib	Aac6′Ib-F	`ACTGAGCATGACCTTGCG`	AY878717
	Aac6′Ib-R	`TGTTCGCTCGAATGCCTG`
bla_IMP	Imp-F	`GTTTTATGTGTATGCTTCC`	AB184976
	Imp-R	`AGCCTGTTCCCATGTAC`
	Imp-out-R	`CCTTCTTCAAGCTTCTCG`
3′ conserved segment region	Qac-F	`TCGCAATAGTTGGCGAAG`	U49101
	Qac-R	`AGCTTTTGCCCATGATGC`
	Sul-F	`GACGGTGTTCGGCATTCT`
	Sul-R	`TGAAGGTTCGACAGCACG`
	Orf5-F	`GGTGATATCGACGAGGTT`
	Orf5-R	`GATTTCGAGTTCTAGGCG`
bla_IMP-19 cloning primers	Sub-IMP19-EcoRI-F	`GGGGAATTCTTAGAAAAGGGCAAGTATG`
	Sub-IMP19-XbaI-R	`GGGTCTAGATCACCGCCTTGTTAGAAAT`