TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| Strains | ||
| PT5 (PAO1) | Wild type | Laboratory collection |
| PT1300 | PT5(pEX1.8) Cbr | This study, 22 |
| PT1308 | PT5(pEXERM) Cbr | This study |
| 11B | PAO1 mexY::Tn501 Hgr | 23 |
| 12B | PAO1 mexZ::bla Cbr | P. Plésiat |
| PT712 | PT5 rhlA::Gm Gmr | 19 |
| PT1323 | PT712(pAKRHL) Cbr | This study |
| PT1332 | PT712(pAK1900) Cbr | 14 |
| Plasmids | ||
| pMLS001 | pMMB206 carrying ermBP, Cmr Eryr | 15, K. Poole |
| pEX1.8 | Cloning vector, Cbr | 22 |
| pAK1900 | Cloning vector, Cbr | 14 |
| pEXERM | pEX1.8 carrying ermBP from pMLS001, Cbr | 15 |
| pAKRHL | pAK1900 carrying rhlABRI from pJPP6, Cbr | 4, 14 |