TABLE 6.
Physiological cost associated with the Q66K alteration in C. trachomatis L2 ribosomal protein L4
| Strain | Mutation | Plaque size (mm)c | EB generation rate (fold increase)d |
|---|---|---|---|
| CtL2 | Wild-type | 0.87 ± 0.24 | 258 ± 47 |
| L2AZM#23a | rplD C196A (K66Q) | 0.21 ± 0.12 | 126 ± 26 |
| L2AZM#31b | rplD C196A (K66Q) | 0.47 ± 0.18 | 241 ± 59 |
a
Twenty-three passages on various concentrations of AZM (up to 50 ng/ml).
b
Twenty-eight passages with up to 100 ng/ml AZM followed by three expansions without AZM.
c
The size of a minimum of 150 individual plaques was determined for each strain in the absence of antibiotic at 14 days p.i. and averaged.
d
The number of PFU produced at 46 h p.i divided by the number used at the time of infection.