Figure 2.

The 500-MHz ¹H and HSQC NMR spectra of covalent Adriamycin-DNA adducts at 10°C. (a) Comparison of CH₂O- and Tris/Fe(III)/DTT-mediated Adriamycin-DNA adducts by ¹H NMR indicates that the two are structurally equivalent. (b) Portion of the carbon HSQC spectrum of ¹³CH₂O-mediated Adriamycin-DNA. The methylene ¹³C has strong cross-peaks to each of its nonequivalent geminal protons, which in turn bear cross-peak arrays structurally consistent with the incorporation of CH₂O into the Adriamycin-DNA adduct as shown in Fig. 1.