Figure 4.

Results of kinetic strand-exchange experiments with unintercalated DNA (○), purified Adriamycin-DNA adduct (□), and DNA intercalated with 1 Adriamycin/duplex. The latter is shown as a dashed line and represents the rate estimated by interpolation from a separate series of strand-exchange experiments containing various concentrations of noncovalently bound Adriamycin. Strand-exchange rate constants are summarized in Table 1. The DNA used in the kinetic strand-exchange experiments was the 14-bp oligomer d(TAATAAGCATAAAT)⋅d(ATTTATGCTTATTA), which contains a single potential site for adduct formation.