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. 1998 Sep 29;95(20):11561–11565. doi: 10.1073/pnas.95.20.11561

Figure 5.

Figure 5

Mediation of stable Adriamycin-DNA adduct formation by glyceraldehyde. Reactions were in buffer A (13), and resolution was on gel type B (13). Reactions contained 100 μM Adriamycin and 100 μM oligomer d(AAAAGCTTTT)2 and were run overnight at 25°C. Lanes: 1, only DNA and Adriamycin; 2, DNA, Adriamycin, and 0.4% CH2O; 3, DNA, Adriamycin, and 0.5% glyceraldehyde. The gel was photographed by UV shadowing. Although not as efficient an initiator of stable Adriamycin-DNA adducts as CH2O, glyceraldehyde mediates stable adduct formation in high enough yield to be of potential biological significance.