TABLE 1.
Mutants selected in 8325-4 as FA resistant and then screened for SCV phenotypes
| Strain and stock | Identified mutationa
|
MIC (μg/ml) of:
|
Doubling time (min)b | Auxotrophyc | nd | |||
|---|---|---|---|---|---|---|---|---|
| fusA (EF-G) | rplF (L6) | FA | STR | KAN | ||||
| 8325-4 | None | None | 0.125 | 3 | 1.5 | 24 | − | |
| FusA classic | ||||||||
| AH207 | Thr385Asn | None | 4 | 12 | 3 | 29 | − | |
| AH194 | Pro404Leu | None | 4 | 8 | 2 | 25 | − | 2 |
| AH196 | Pro406Leu | None | 6 | 8 | 3 | 31 | − | |
| AH198 | Gly452Ser | None | 32 | 6 | 4 | 38 | − | 2 |
| AH201 | Gly452Cys | None | 12 | 8 | 6 | 28 | − | |
| AH197 | Leu456Phe | None | 4 | 8 | 6 | 34 | − | |
| AH187 | His457Tyr | None | 96 | 6 | 3 | 29 | − | 9 |
| AH206 | Arg464Ser | None | 6 | 8 | 6 | 37 | − | |
| AH190 | Arg464His | None | 6 | 8 | 4 | 31 | − | 3 |
| FusA-SCV | ||||||||
| AH188 | Gly664Ser | None | 4 | 16 | 24 | 46 | Hemin | 2 |
| FusE | ||||||||
| AH193 | None | TAA stop at nt 229 | 4 | 24 | 8 | 54 | Hemin | |
| AH199 | None | TAA stop at nt 241 | 4 | 16 | 8 | 57 | Hemin | |
| AH202 | None | CTG start at nt 1 | 4 | 16 | 8 | 58 | Hemin | |
| AH204 | None | Δ of nt 203-231 | 4 | 24 | 8 | 57 | Hemin | |
| AH209 | None | Δ of nt 404-427 | 4 | 16 | 8 | 60 | Hemin | |
a
None, wild type. Underlined letters represent the mutated residues.
b
Doubling time (generation time) in minutes during exponential growth in LB.
c
Auxotrophy tested for hemin, menadione, thymine, and thymidine. −, nonauxotrophic.
d
Number of independent isolates with the same genotype and phenotype.