TABLE 1.
Strain and stock | Identified mutationa
|
MIC (μg/ml) of:
|
Doubling time (min)b | Auxotrophyc | nd | |||
---|---|---|---|---|---|---|---|---|
fusA (EF-G) | rplF (L6) | FA | STR | KAN | ||||
8325-4 | None | None | 0.125 | 3 | 1.5 | 24 | − | |
FusA classic | ||||||||
AH207 | Thr385Asn | None | 4 | 12 | 3 | 29 | − | |
AH194 | Pro404Leu | None | 4 | 8 | 2 | 25 | − | 2 |
AH196 | Pro406Leu | None | 6 | 8 | 3 | 31 | − | |
AH198 | Gly452Ser | None | 32 | 6 | 4 | 38 | − | 2 |
AH201 | Gly452Cys | None | 12 | 8 | 6 | 28 | − | |
AH197 | Leu456Phe | None | 4 | 8 | 6 | 34 | − | |
AH187 | His457Tyr | None | 96 | 6 | 3 | 29 | − | 9 |
AH206 | Arg464Ser | None | 6 | 8 | 6 | 37 | − | |
AH190 | Arg464His | None | 6 | 8 | 4 | 31 | − | 3 |
FusA-SCV | ||||||||
AH188 | Gly664Ser | None | 4 | 16 | 24 | 46 | Hemin | 2 |
FusE | ||||||||
AH193 | None | TAA stop at nt 229 | 4 | 24 | 8 | 54 | Hemin | |
AH199 | None | TAA stop at nt 241 | 4 | 16 | 8 | 57 | Hemin | |
AH202 | None | CTG start at nt 1 | 4 | 16 | 8 | 58 | Hemin | |
AH204 | None | Δ of nt 203-231 | 4 | 24 | 8 | 57 | Hemin | |
AH209 | None | Δ of nt 404-427 | 4 | 16 | 8 | 60 | Hemin |
None, wild type. Underlined letters represent the mutated residues.
Doubling time (generation time) in minutes during exponential growth in LB.
Auxotrophy tested for hemin, menadione, thymine, and thymidine. −, nonauxotrophic.
Number of independent isolates with the same genotype and phenotype.