TABLE 1.
Plasmids and strains used in this study
| Plasmid or strain | Description | Reference |
|---|---|---|
| Plasmids | ||
| pPR9TT | Translational fusion LacZ reporter vector | 31 |
| pNJH123 | pPR9TT-PurcAlacZ reporter vector | This study |
| pBAD-GFP | Prokaryotic GFPuv expression vector | 8 |
| pRVYFP-2 | Low-copy-number expression vector | M. Thanbichler, unpublished |
| pNJH193 | pRVYFP-2-PurcAgfpuv reporter vector | This study |
| pNJH198 | pRVYFP-2-PurcA His6gfpuv reporter vector | This study |
| pNJH200 | pRVYFP-2-PurcA_RBS His6gfpuv reporter vector | This study |
| pBVMCS-2 | High-copy-number expression vector | M. Thanbichler, unpublished |
| pNJH201 | pBVMCS-2-PurcA_RBS His6gfpuv reporter vector | This study |
| pX31 | High-copy-number xylose-inducible expression vector | A. Iniesta, unpublished |
| pNJH153 | pX31-Pxylgfpuv expression vector | This study |
| pRSET-B-mCherry | mCherry expression vector | 32 |
| pMT383 | pPvan-ftsZ-eyfp expression integration vector | 35 |
| pNJH15 | pPvan-ftsZ-mcherry expression integration vector | This study |
| pMT397 | Low-copy-number pPvan-MCS-eYFP expression vector | M. Thanbichler, unpublished |
| pNJH156 | pX31-Pxylgfpuv (or eyfp) expression vector | This study |
| pNJH169 | pX31-PxylurcA-mcherry expression vector | This study |
| Strains | ||
| LS4358 | C. crescentus CB15N ΔCC_1634 | J. C. Chen, unpublished |
| NJH199 | C. crescentus CB15N ΔCC_1634(pNJH123) | This study |
| LS101 | C. crescentus CB15N | 11 |
| NJH371 | C. crescentus CB15N(pNJH201) | This study |
| NJH250 | C. crescentus CB15N(pNJH153) | This study |
| NJH300 | C. crescentus CB15N(pNJH169) | This study |