TABLE 3.
Differentiation of S. enterica subpopulations and serotypes by antibiotic compounds that screen for dimorphism
| Compound name | Concn range of antibiotic (μg/ml) | PM plate no. |
P of similarity to wt serotype Enteritidis PT13aa
|
||||
|---|---|---|---|---|---|---|---|
| BF serotype Enteritidis PT13a | Serotype Enteritidis PT4 | Serotype Typhimurium | Serotype Heidelberg | Serotype Newportb | |||
| 2,4-Diamino-6,7-diisopropylpteridine | 0.7-70 | 12B | 0.353 | 0.001 | 0.000 | 0.001 | 0.003 |
| 3,5-Diamino-1,2,4-triazole (guanazole) | 83-8,300 | 18C | 0.076 | 0.000 | 0.000 | 0.000 | 0.000 |
| 5-Fluoroorotic acid | 1-100 | 12B | 0.418 | 0.000 | 0.001 | 0.000 | 0.003 |
| 5-Fluoro-5′-deoxyuridine | 0.7-70 | 18C | 0.007 | 0.000 | 0.000 | 0.002 | 0.000 |
| β-Chloro-l-alanine | 26-2,600 | 17A | 0.268 | 0.000 | 0.020 | 0.000 | 0.000 |
| Azathioprine | 6-600 | 18C | 0.043 | 0.000 | 0.000 | 0.000 | 0.000 |
| Cefamandole | 0.03-3 | 17A | 0.171 | 0.000 | 0.000 | 0.000 | 0.000 |
| Cefsulodin | 0.9-90 | 17A | 0.120 | 0.000 | 0.000 | 0.000 | 0.000 |
| Ceftriaxone | 0.002-0.2 | 11C | 0.355 | 0.000 | 0.001 | 0.000 | 0.000 |
| Cesium chloride | 14-1,400 | 13B | 0.838 | 0.001 | 0.004 | 0.003 | 0.002 |
| Cetoperazone | 0.007-0.7 | 17A | 0.960 | 0.000 | 0.000 | 0.000 | 0.000 |
| Chelerythrine | 0.6-60 | 14A | 0.598 | 0.000 | 0.000 | 0.000 | 0.000 |
| Chlorhexidine | 0.006-0.6 | 19 | 0.013 | 0.000 | 0.000 | 0.000 | 0.000 |
| Chromium chloride | 6-600 | 16A | 0.009 | 0.000 | 0.000 | 0.000 | 0.000 |
| d-Cycloserine | 0.3-30 | 15B | 0.613 | 0.000 | 0.011 | 0.000 | 0.000 |
| Diamide | 2.6-260 | 16A | 0.530 | 0.009 | 0.011 | 0.010 | 0.004 |
| Erythromycin | 0.7-70 | 11C | 0.047 | 0.002 | 0.030 | 0.000 | 0.016 |
| Fusidic acid | 11-1,100 | 15B | 0.041 | 0.000 | 0.002 | 0.000 | 0.000 |
| Glycine | 36-3,600 | 13B | 0.001 | 0.000 | 0.000 | 0.000 | 0.000 |
| l-Glutamic-γ-hydroxamate | 0.5-50 | 16A | 0.082 | 0.000 | 0.000 | 0.000 | 0.000 |
| Moxalactam | 0.01-1 | 13B | 0.024 | 0.000 | 0.000 | 0.000 | 0.000 |
| Myricetin | 2-200 | 18C | 0.261 | 0.000 | 0.011 | 0.003 | 0.001 |
| Nitrofurantoin | 0.08-8 | 14A | 0.100 | 0.000 | 0.013 | 0.000 | 0.002 |
| Oleandomycin | 13-1,300 | 15B | 0.053 | 0.002 | 0.466 | 0.001 | 0.036 |
| Oxolinic acid | 0.01-1 | 13B | 0.812 | 0.000 | 0.000 | 0.000 | 0.000 |
| Phosphomycin | 0.17-17 | 16A | 0.256 | 0.000 | 0.000 | 0.000 | 0.000 |
| Piperacillin | 0.07-7 | 14A | 0.030 | 0.000 | 0.000 | 0.000 | 0.000 |
| Rifamycin SV | 3-300 | 16A | 0.650 | 0.000 | 0.456 | 0.000 | 0.000 |
| Sodium bromate | 210-21,000 | 18C | 0.027 | 0.005 | 0.056 | 0.003 | 0.001 |
| Sorbic acid | 15-1,500 | 16A | 0.000 | 0.000 | 0.010 | 0.000 | 0.000 |
| Spectinomycin | 0.3-30 | 12B | 0.087 | 0.001 | 0.000 | 0.000 | 0.000 |
| Sulfanilamide | 3.6-360 | 16A | 0.596 | 0.005 | 0.020 | 0.003 | 0.000 |
| Tinidazole | 11-1,100 | 18C | 0.020 | 0.000 | 0.405 | 0.300 | 0.132 |
| Triclosan | 0.023-2.3 | 18C | 0.236 | 0.000 | 0.000 | 0.000 | 0.000 |
| Trifluoperazine | 6-600 | 13B | 0.054 | 0.001 | 0.095 | 0.000 | 0.005 |
| Trifluorothymidine | 0.07-7 | 18C | 0.040 | 0.000 | 0.000 | 0.000 | 0.000 |
a
A P of <0.01 indicates a significant difference.
b
Serotype Newport has multiple antibiotic resistances; thus, it has an origin of antibiotic resistance that is fundamentally different to that of serotype Enteritidis that correlates with single-nucleotide polymorphisms.