FIG. 3.

Effects of osmolarity on in vivo-induced gene fusions. Strains carrying a fusion of the indicated gene to lacZ were grown as standing overnight cultures in MOPS minimal medium (gray bars) or with the addition of 0.4 M NaCl (black bars). Triplicate cultures of each strain were assayed for lacZ expression in CRPG-enhanced β-galactosidase assays. All fusions shown, except for STM2689, produced a significant increase (P ≤ 0.05) in expression due to the addition of the NaCl. STM2689 is shown as representative of fusions that were not induced under these conditions. The β-galactosidase concentration was calculated as defined in Materials and Methods. Error bars show standard deviations.