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(A) Detection of mIL-2 in culture supernatant of L. lactis FI9957 by Western blot analysis. Lane 1, FI9957; lane L, MagicMark Western protein standard. (B) Slot blot detection of mIL-2 in the culture supernatants of L. lactis strains. Row I, nisin induced (50 ng ml−1); row U, not induced with exogenous nisin. Lane 1, FI7847; lane 2, FI9947; lane 3, FI9949; lane 4, murine IL-2 (2 ng ml−1); lane 5, murine IL-2 (0.2 ng ml−1); lane 6, FI5876; lane 7, FI9955; lane 8, FI9957.
