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. 2007 Oct 12;73(23):7622–7628. doi: 10.1128/AEM.01000-07

FIG. 6.

FIG. 6.

The prtS gene is transcribed during infection of M. sexta. (A) Growth of P. luminescens TT01 in M. sexta. M. sexta hemocoel was injected with either 10 CFU P. luminescens TT01 or 10,000 CFU E. coli VCS257. At indicated time points postinjection, 100 μl of hemolymph was collected from the horn of the insect and dilutions were made in PBS and plated to obtain colony counts. At 48 h postinjection with P. luminescens, the M. sexta died. (B) Detection of prtS transcript using RT-PCR. Hemolymph samples from M. sexta were collected as described above, total RNA was isolated, and RT-PCR was performed as described in Materials and Methods. Samples from three M. sexta insects per time point were examined. Hours postinjection are indicated above the horizontal lines. “0” represents hemolymph sample immediately after injection; − represents samples from uninfected controls.