TABLE 1.

Peptides found in native gels with preparations of membrane proteins and whole cells

Locus tag	GenBank accession no.	No. of peptides found (gel 1/gel 2)a	Mascot score(s) (gel 1/gel 2)	No. of matched m/z values (gel 1/gel 2)	No. of matching MS/MS spectra (gel 1/gel 2)	% of sequence coverage (gel 1/gel 2)	Annotation	Gene	Calculated mass (kDa)b	Presence of signal peptide (name)
cbdbA84	CAI82345	1/2	263/277	24/28	2/6	50/52	Chlorobenzene reductive dehalogenase	cbrA	51.2	Yes (Tat)
cbdbA80	CAI82340	1	162	11	2	25	Putative reductive dehalogenase	rdhA	52.5	Yes (Tat)
cbdbA195	CAI82443	1/2*	165/662	10/16	1/15	14/21	Formate dehydrogenase, major subunit	fdnG	102.2	Yes (Tat)
cbdbA131	CAI82389	2*	164	4	3	15	Putative Ni/Fe hydrogenase, iron-sulfur cluster-binding subunit		30.4	No
cbdbA1368	CAI83414	1/2*	111/255	4/7	1/6	8/11	Conserved domain protein (putative S-layer protein [24])		103.7	Yes
cbdbA1039	CAI83145	1	310	20	2	37	ABC-type dipeptide/oligopeptide/nickel transport system, periplasmic component		58.4	Yes
cbdbA1463	CAI83489	2	367	10	5	25	Peptide ABC transporter, periplasmic peptide-binding protein		56.8	Yes
cbdbA1393	CAI83436	1	171	3	3	7	Co-chaperonin GroEL	groEL	57.1	No
cbdbA960	CAI83087	2*	164	4	4	14	Translation elongation factor Tu	tuf	44.0	No
cbdbA1358	CAI83407	2*	460	11	10	21	Chaperone protein DnaK	dnaK	68.5	No
cbdbA1675	CAI83683	2*	268	8	6	43	LemA family protein		18.9	Yes
cbdbA1077	CAI83178	2*	420	17	15	21	CoA binding ligase		97.9	No
cbdbA1729	CAI83727	2*	73	5	3	15	TPR domain protein		30.0	No

^aPeptides were detected with preparations of membrane proteins (gel 1) and whole cells (gel 2). *, peptides identified by nano LC-MALDI MS. Threshold score (P < 0.05) = 44.

^bTheoretical masses of proteins with leader peptides are given for the processed form. Tat, twin-arginine signal peptide.