FIG. 3.

Effect of temperature on LipA (○) and LipB (▪) activities. The lipase assay was conducted as mentioned in Materials and Methods. The substrate used for the assay was p-NPL. A similar temperature profile but lower specific activity was also obtained when p-NPL was replaced with p-NPP. The insert is the temperature profile when only TAPS buffer was used at pH 9.0. A true substrate, trioleate, was used at temperatures of ≥98°C, and the liberated fatty acids were quantified by using an NEFA C kit. The 100% relative activities were 11.8 ± 0.5 U mg⁻¹ and 13.0 ± 0.6 U mg⁻¹ for LipA and LipB, respectively. The activity values were corrected for control values obtained without enzyme addition. Error bars show standard deviations.