FIG. 1.
Total (solid bars) and adherent (open bars) cell populations of the wild-type strain (EGD/pTV), mutant strains (3A6/pTV and 3A10/pTV), and the lmo1462-complemented mutant strains (3A6/pTV1462 and 3A10/pTV1462). One-hundred-microliter inocula containing 5 × 107 CFU of each strain were deposited on clean, sterile, stainless steel slides which were placed at 30°C for 24 h and then at 3°C for 6 days for biofilm formation. Nonadherent (planktonic) and adherent bacterial cells were counted. The sum of adherent and planktonic CFU is referred to as the “total population.” Bars indicate the grand means of mean values obtained from one triplicate and two single independent experiments (n = 3) in the case of 3A6, from three triplicate independent experiments (n = 3) in the case of 3A10, and from four independent triplicate experiments (n = 4) in the case of the wild-type strain. Error bars represent standard deviations (no error bar is shown for 3A6/pTV1462 total population as all values were identical).