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SDS-PAGE protein analysis during purification of recombinant ht-PhnII from strain KT2442 carrying pVEHD11. Lane 1, protein markers; lane 2, soluble cell extract; lane 3, DEAE column eluate; lane 4, IMAC column eluate; lane 5, dialyzed purified protein. The arrows indicate the α and β subunits of ht-PhnII.
