Time-lapse analysis of expression of chiAC in recombinant B. sphaericus 2297-pBbC by SDS-PAGE (A) and Western blotting (B). Lane M, markers; lanes 1 to 13, bacterial cellular extracts taken from the cultures at 4, 8, 12, 16, 20, 24, 32, 40, 48, 56, 64, 72, and 80 h.