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. 1999 Dec 13;147(6):1237–1248. doi: 10.1083/jcb.147.6.1237

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Localization of AE1 anion exchangers and the H⁺-ATPase in the cells of the chicken kidney collecting duct. The kidney from a 17-d-old chicken embryo was fixed, and frozen in embedding media. 3-μm tissue sections were incubated with a polyclonal antibody specific for the chicken AE1 anion exchanger, and with a mouse monoclonal antibody specific for the H⁺-ATPase. Immunoreactive polypeptides were detected with DAR-IgG conjugated to lissamine and DAM-IgG conjugated to FITC, and visualized on a BioRad laser scanning confocal microscope. The 0.5-μm confocal images showing the distribution of AE1 (A) and the H⁺-ATPase (B) were merged (C) in Adobe Photoshop. The arrow denotes a cell with both basolateral and apical AE1 and apical H⁺-ATPase.